Time-Resolved Single Molecule Microscopy Coupled with Atomic Force Microscopy
نویسندگان
چکیده
منابع مشابه
Single Molecule Force Spectroscopy on Polysaccharides by Atomic Force Microscopy
Recent developments in piconewton instrumentation allow the manipulation of single molecules and measurements of intermolecular as well as intramolecular forces. Dextran filaments linked to a gold surface were probed with the atomic force microscope tip by vertical stretching. At low forces the deformation of dextran was found to be dominated by entropic forces and can be described by the Lange...
متن کاملSingle Molecule DNA Biophysics with Atomic Force Microscopy
Structural and functional properties of double stranded deoxyribonucleic acid (dsDNA) are investigated by atomic force microscopy (AFM) on a single molecule level. Here, we characterize different linear and circular DNA systems in terms of their geometry and topology, and visualize enzyme binding of restriction endonuclease Hae III to DNA. Manipulation of single DNA molecules is demonstrated by...
متن کاملHigh-Resolution Correlative Microscopy: Bridging the Gap between Single Molecule Localization Microscopy and Atomic Force Microscopy.
Nanoscale characterization of living samples has become essential for modern biology. Atomic force microscopy (AFM) creates topological images of fragile biological structures from biomolecules to living cells in aqueous environments. However, correlating nanoscale structure to biological function of specific proteins can be challenging. To this end we have built and characterized a correlated ...
متن کاملSingle molecule study of xanthan conformation using atomic force microscopy.
Conformations of individual macromolecules of the biopolymer xanthan were investigated using atomic force microscopy (AFM). Xanthan from very dilute solutions (1 ppm) was allowed to adsorb onto freshly cleaved mica and examined using tapping mode AFM under ambient conditions. The secondary structure of xanthan was probed by heating the polymer and gradually cooling, which denatured and renature...
متن کاملProbing single biomolecules with atomic force microscopy.
During the last years, atomic force microscopy (AFM) has developed from a microscopy tool for solid-state surface science toward a method employed in many scientific disciplines, such as biology, for investigating individual molecules on a nanometer scale. This article describes the current status of the imaging possibilities of AFM on RNA, IgG, and gold-labeled cell adhesion proteoglycans, as ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.3198